| | Category | ME | L24 | Comparative Significance of Cell Spreading and Division in |
| | Monolayer Reform |
| | Abstract | The reformation of a contiguous cell monolayer is a multifaceted process |
| | that involves an intricate coordination of two general processes: cell |
| | division and cell spreading. In response to interruptions in a cell monolayer, |
| | cell division, a well-studied process employed by cells to reproduce, |
| | works in conjugation with cell spreading, a gradual flattening and |
| | locomotion of cells, to restore lost contiguity. |
| | This study focuses on the comparative significance of cell division and cell |
| | spreading in monolayer restoration. Monolayers of PtK2 Potorous Kidney |
| | Epithelial Cells were treated with the drugs Aphidicolin and EHT-1864 to |
| | selectively inhibit cell division and cell spreading, respectively, and were |
| | struck to introduce a disruption in contiguity. The initial rate of monolayer |
| | restoration in non-spreading monolayers was 71% slower than in non- |
| | dividing monolayers. However, over a 65 hour period, the reformation rate |
| | in non-spreading monolayers doubled while the rate halved in non-dividing |
| | monolayers. Interestingly, a stain analysis of intracellular dynamics |
| | indicated an upregulation of microfilament production in non-dividing |
| | monolayers while incidence of mitoses remained constant between non- |
| | spreading monolayers and the control. |
| | The results suggest that cell spreading is a key component in initial |
| | monolayer restoration while cell division requires time to substantially |
| | contribute to reformation. Also, the inhibition of cell division appears to |
| | promote cell spreading, but not vice versa. Monolayer reformation, |
| | although an intricate process in itself, plays an integral role in other, more |
| | complex process. Studies of monolayer reformation are often incorporated |
| | in wound surrogacy analyses and have numerous implications in wound |
| | healing, ulcer treatment, and skin cancer care. |
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